Virus-induced gene complementation in tomato
نویسندگان
چکیده
منابع مشابه
Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening
Plant virus technology, in particular virus-induced gene silencing, is a widely used reverse- and forward-genetics tool in plant functional genomics. However the potential of virus technology to express genes to induce phenotypes or to complement mutants in order to understand the function of plant genes is not well documented. Here we exploit Potato virus X as a tool for virus-induced gene com...
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Background and Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense mechanism. By infecting plants with recombinant viruses containing host genes inserted in the viral genome, VIGS achieves the RNA silencing process. The purpose of this study was to investigate the possibility of tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana be...
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Virus-induced gene silencing (VIGS) is a valuable tool for identification and characterization of genes function. To improve the efficiency of VIGS in different organ, we developed an organ-specific VIGS that could be applied to tomato (Solanum lycopersicum cv Micro Tom) leaves, flowers and fruits respectively. With phytoene desaturase (PDS) as a reporter gene, almost up to 100% of efficiency o...
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RNA interference (RNAi) is a highly specific gene-silencing phenomenon triggered by dsRNA. This silencing mechanism uses two major classes of RNA regulators: microRNAs, which are produced from non-protein coding genes and short interfering RNAs (siRNAs). Plants use RNAi to control transposons and to exert tight control over developmental processes such as flower organ formation and leaf develop...
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Lycopene biosynthesis in tomato (Solanum lycopersicum) fruits has been proposed to proceed through a poly-cis pathway catalyzed by phytoene synthase (PSY), two desaturases (phytoene desaturase [PDS] and ζ-carotene desaturase [ZDS]), and two cis-trans isomerases (ζ-carotene isomerase [ZISO] and prolycopene isomerase [CrtISO]). The mechanism of action of these enzymes has been studied in Escheric...
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ژورنال
عنوان ژورنال: Plant Signaling & Behavior
سال: 2013
ISSN: 1559-2324
DOI: 10.4161/psb.27142